一 , 課程說明(Course Description)

分析共軛焦顯微鏡技術原理，生物標本製備練習，共軛焦顯微鏡操作，3D 生物影像處理。並邀請軟、硬體工程師及在生物影像領域研究成果卓著的老師們共同指導。

二 , 指定用書(Text Books)
Alan R. Hibbs (2004) Confocal Microscopy for Biologists. Amazon

三 , 參考書籍(References)

Alberto Diaspro (2002) Confocal and Two-Photon Microscopy : Foundations, Applications and Advances
Brian Matsumoto (2002) Cell Biological Applications of Confocal Microscopy, Second Edition (Methods in Cell Biology)
Beesley, J. E. (1993) Immunocytochemistry, A Practical Approach. IRL press.
Lacey, A. J. (1989) Light Microscopy in Biology, A Practical Approach. IRL press, New York.
Slayter, E. M. and Slayter H. S. (1992) Light and Electron Microscopy. Cambridge University Press, New York.
Stevens, J.K., Milles, L.R. and Trogadis, J. E. (1994) Three-dimensional confocal microscopy of biological specimens. London, Academic Press.
Taylor, D. L. and Wang, Y.-L. and (1989) Fluorescence Microscopy of Living Cells in Culture, Part B. Quantitative Fluorescence Microscopy Imaging and Spectroscopy. Academic Press. New York.
Wang, Y.-L. and Taylor, D. L. (1989) Fluorescence Microscopy of Living Cells in Culture, Part A. Fluorescent Analogs, Labeling Cells, and Basic Microscopy. Academic Press. New York.

四、教學方式(Teaching Method)

Interactive lecture, discussion, pratical training

五、教學進度(Syllabus)

9/6 ( 三 )
10:00-12:00 光學顯微鏡與螢光顯微鏡基本原理 (台灣儀器工程師 )
13:10-16:00 光學顯微鏡與螢光顯微鏡操作 (台灣儀器工程師 )

9/7 ( 四 )
10:00-12:00 光學顯微鏡與螢光顯微鏡操作 (台灣儀器工程師 )
13:10-16:00 共軛焦顯微鏡進階操作 (台灣儀器工程師 )

9/8 ( 五 )
9:30-12:30 Amira3D影像視覺化軟體訓練課程I (Amira工程師 )
13:30-16:30 Amira3D影像視覺化軟體訓練課程II (Amira工程師 )

9/11 Confocal microscopy overview (江安世老師)

911-9/15 Amira practice (張修明博士)
繳交自行分組名單（四人/組）給腦科學中心助理 林薏欣 #42478，安排操作時間至腦科學中心練習，每組約3小時。

9/18 FLIM/FRET_ a novel tool for molecular dynamics imaging (高甫仁老師)

9/18-10/20 分組至腦科學中心練習『虛擬顯微鏡操作』and LSM 510 software。每組約6小時。

10/23 Multicolor imaging (鄧淑方)

10/30 Imaging gene expression patterns reported by GFP (許庭嘉) (shaker-GFP x 247-DsRed)

11/6 Immunohistochemistry and GFP double labeling (陳怡君)

11/13 3D Image Processing Techniques for Confocal Images (陳永昌老師)

11/20 Neural network analysis (荊宇泰老師)

11/27 Bioimaging overview (胡宇光老師)

11/27-12/15 LSM practice and term paper preparation
Make appointment with 許庭嘉 or 陳怡君 if you need practice

12/18 影像資料庫及展示系統 (謝昌煥老師)

12/25 LSM operation test (鄧淑方) & Term paper due (林薏欣)

六、成績考核(evaluation)
Discussion 20%
LSM operation test 40%
Term paper 40%