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The Bioimaging Core at Tsinghua Brain Research Center (BRC) in the University System of Taiwan (UST) comprises several groups of research faculty involved in cell biological and molecular brain imaging, opotelectronic design, and computer/imaging science. The Bioimaging Core has initiated a collaboration between researchers at the National Tsing Hua University as well as UST. As a service to the scientific community, the Bioimaging Core provides access to it imaging and computer hardware.
For more information email the Bioimaging Core or phone 03-5715131-2478
Facilities
ĦE Zeiss LSM 510 confocal microscope mounted on an inverted microscope (Axiovert 135) is equipped with 4 laser light sources including an argon laser (emission at 364 nm), an argon-krypton laser (458, 488, or 514 nm), and two HeNe lasers (543 and 633 nm). The system allows for simultaneous detection of four fluorescence signals and a transmitted image. This is designed for routine observation of fixed or live single cells on slides or culture chambers. (funded by NSC)
ĦE Zeiss LSM 510 META confocal two-photon microscope system mounted on an upright microscope (Axioplan 2) is equipped with 4 laser light sources including an argon-krypton laser (458, 488, or 514 nm), two HeNe lasers (543 and 633 nm), and with a Coherent Mira femtosecond T-Sapphire laser for nonlinear optical microscopy (2-photon) which is capable of 700-1000nm single optics set tuning. This is designed for in vivo observation of fluorescence signals in thick living tissues. (funded by BRC)
ĦE Zeiss LSM 510 META confocal microscope mounted on an upright microscope (Axioplan 2) is equipped with 3 laser light sources including an argon-krypton laser (458, 488, or 514 nm), and two HeNe lasers (543 and 633 nm). The system has 3 photomultipliers and a META detector allowing simultaneous collection of full spectrum fluorescence signals. It does not have transmitted light detector. It has an automated stage scanner for image montage and optical system for IR light. This is designed for instrument development of novel two-photon system. (funded by NSC)
Bioimaging Core Policies
ĦE BIOIMAGING CORE IS NOT RESPONSIBLE FOR DATA STORAGE. Users should remove data from bioimaging computers immediately to avoid possible loss. A variety of storage options are available.
ĦE Use of the microscopes and imaging workstations is restricted to authorized users. To become an authorized user, you must receive a training session from the Bioimaging Core staff for each piece of equipment used. In addition, use of the Zeiss LSM510 confocal microscope is retricted to appropriate projects and must be approved by the Deputy Director (see LSM 510 Meta Two photon User Policy). If you do not wish to become a user, you may arrange to work with a bioimaging staff person at the hourly rate.
ĦE Assistance from Bioimaging Core staff will be charged at the hourly rate. Please see Fee Schedule for our current rates.
Microscope Policies (additional information)
ĦE Please do not sign up for more than 4 hours at one session unless you have requested additional time from Bioimaging Core Staff (03-5715131-2478). Normally, additional time will be allotted for live cell experiments or for outside collaborations. You may sign up for an additional 4 hours in the same week, but you may be bumped.
ĦE If you cannot keep your appointment, please delete your name from the sign up and call the person signed up before you (or bioimaging core Staff) to let them know that they may have additional time.
ĦE Please try not to sign up for time unless you know you will use it. It is understandable that some experiments will fail or other problems might arise. However, given the high demand for these instruments, it is inconsiderate to other researchers to consistently sign up and then cancel sessions at the last minute.
ĦE After 5 pm, unless you know for certain that someone else is using the microscope, please shut down the instrument.
ĦE After work hours and weekend use is available for trained, competent users. Please check with bioimaging core Staff prior to signing up.
Fee Schedule for Bioimaging Core 2/20/2004
| Description |
Academic Rates |
Industrial Rates |
| Microscope Use |
$1,000/PI/year |
$3,000/PI/year |
| Computer Workstations only |
$500/PI/year |
$1,000/PI/year |
| **Complete Package** |
$1,200/PI/year |
$3,500/PI/year |
| Service Work by BRC Staff |
$50/hour |
$150/hour |
| Microscope Training Session |
$150 |
$300 |
| Prints from dye-sublimation printer |
$5.00/page |
$5.00/page |
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This package includes the use of the microscopes, computer workstations and the Molecular Core resources for 1 year. This package does not include the training fees.**
The Microscope account allows access to the Bioimaging core microscopes. You must be an authorized user, trained by the Bioimaging core staff, to use each microscope. The fee for the training session is a one time fee, however there is a training session for each microscope. If you do not wish to become a user, you may arrange to work with a BRC staff person at the hourly rate. Please see Bioimaging microscope user policies for more information.
The Computer Workstations account provides access to BRC software (including LSM510 and Amira 3.1) for individuals who do not require use of the microscopes.
Industrial Partners
Bioimaging Core encourages a variety of interactions with imaging and biomedical-related industries. In addition to formal research contracts and consulting arrangements, Tsing Hua Bioimaging Core offers an Industrial Associates Program that provides access to Bioimaging core facilities, seminars, and workshops as well as informal consulting with staff and faculty. Contact Bioimaging Core for more information.
Microscope User Policies
The following information applies to the microscopes Bioimaging Core currently houses:,the Zeiss LSM 510, the Zeiss LSM 510 META and the LSM510 META Two photons instrument. Please read the additional User Policy notes for the Zeiss LSM 510 META confocal and the Zeiss LSM510 META Two-photons microscope systems.
Individuals who are interested in using any of the microscopes may do so either on a service work basis or you may be trained to use the microscope set-up on your own.
ĦE Service Work: a member of the Bioimaging Core staff will assist you in using the microscope for image acquisition. There is an hourly fee charged for this service, please see the fee schedule for the current rates.
ĦE Microscope Training: a member of the Bioimaging Core staff will train you on a microscope such that you become competent to work independently. Once you are trained to use the microscope independently, you may sign up for the scope during work hours or for after work hours / weekend use.
Please note that you must be trained for each microscope separately. There is a yearly microscope user fee and a one time training fee per microscope. Please see the fee schedule for the current rates.
General Microscope User Guidelines
ĦE Please do not sign up for more than 4 hours at one session unless you have requested additional time from Bioimaging Core Staff (03-5715131-2478). Normally, additional time will be allotted for live cell experiments or for outside collaborations. You may sign up for an additional 4 hours in the same week, but you may be bumped.
ĦE If you cannot keep your appointment, please delete your name from the sign up and call the person signed up before you (or bioimaging core Staff) to let them know that they may have additional time.
ĦE Please try not to sign up for time unless you know you will use it. It is understandable that some experiments will fail or other problems might arise. However, given the high demand for these instruments, it is inconsiderate to other researchers to consistently sign up and then cancel sessions at the last minute.
ĦE After 5 pm, unless you know for certain that someone else is using the microscope, please shut down the instrument.
ĦE After work hours and weekend use is available for trained, competent users. Please check with bioimaging core Staff prior to signing up.
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