Reviewed manuscripts for Science, Current Biology, PNAS, Cell Reports, PLoS Biology, PLoS Genetics, eLife, Journal of Neuroscience, Nature Communications
Reviewed International grants for: National Science Foundation, Science Foundation Ireland, Wellcome Trust (2014, 2015, 2016, 2017), AXA Research Fund (2015), European Research Council (2017)
國際課程講師: National Centre for Biological Sciences, Bangalore, India (2011); Marine Biological Laboratory at Woods Hole (2006); Cold Spring Harbor Laboratory (2002)
MAJOR SCIENTIFIC ACHIEVEMENTS
We aim to understand the genetic and neural basis of brain functions using Drosophila as a model system. We take two main approaches: (i) to construct a wiring diagram of information flows in the entire brain, and (ii) to manipulate target neurons for understanding the computation process in specific neural circuits orchestrating the studied behaviors. Major achievements in our attempts to reach these goals include:
Invent the world-first tissue clearing technology.
We developed an optical tissue clearing solution, called FocusClear (J Comp Neurol 2001, 440:1-11; Methods 2003, 30:86-93), compatible to GFP for the visualization of gene expressions in an intact brain at single cell resolution. Making biological tissue transparent allows efficient excitation and detection of fluorescence signals at the depth hundreds of micron beneath the surface. Combining with confocal microscopy, our tissue clearing technology has revolutionized traditional anatomy by allowing high-resolution 3D imaging of large biological tissues without physical sectioning. The invention has received patents from Taiwan (Patent No: 206390), UAS (Patent No: US 6472216 B1), France (Patent No: 2830323) and United Kingdom (Patent No: GB237757).
Discover odor representation in the higher brain centers via a circuit shunting mechanism. We discovered neuronal temporal identity and stereotyped neural connections in the 3rd order olfactory center of the Drosophila brain (published in Cell 2006, 127:409-422; Cell 2007, 128:1205-1217) and a shunting mechanism for gating CO2 information flow in parallel neural circuits (Science2013, 340:1338-1341). Our findings extend the understanding of olfactory representations to the 3rd order brain center and show that information routing is context dependent.
Discover that long-term memory stores in only few brain neurons. Forming long-term memory requires new protein synthesis in all animals. We found that learning-induced new proteins occur in only few brain neurons for the formation of long-term memory (Science 2012, 335:678-685; Proc Natl Acad Sci USA 2013, 110:7898-7903). Previously, we have established fly models for studying long-term memory formation (Current Biology 2003, 13:286-296), intermediate-term memory consolidation (Current Biology 2011, 21:848-854; Proc Natl Acad Sci USA 2011, 108:13794-13799), aging dependent memory defect (Neuron 2003, 40:1003-1011), Alzheimer’s diseases (Proc Natl Acad Sci USA 2004, 101:6623-6628), Huntington’s diseases (Proc Natl Acad Sci USA 2005, 102:3777-3781), and IBMPFD disease model (PLoS Genetics 2011, 7:e1001288). Importantly, we discovered the first functional NMDA receptors in insects (Proc Natl Acad Sci USA 2002, 99:37-42; Current Biology 2005, 15:603-615; Nature Neuroscience 2007, 10:1578-1586). This series of studies suggest a unified cellular model for learning and memory and extend our understanding of memory formation from molecular to systems consolidation. (Curr Opin Neurobiol 2013, 23:84-91).
Construct the world-first 3D fly brain database at single neuron resolution. We created the first brain-wide wiring map in Drosophila (Current Biology 21:1-11, 2011). The study managed to bar code 16,000 neurons in the fly brain, an advance that is comparable to the sequencing of the fly genome and considered as the first step toward decoding the human brain by The New York Times. The breakthrough paves the way for a comprehensive analysis of information processing within and between neurons and ultimately a deeper understanding of control and causality in fly behavior (Current Biology 2011, 21:R19-20; Proc Natl Acad Sci USA 2014, 111:5367-5372). Guided by this brain circuit map, we have mapped auditory circuit (Proc Natl Acad Sci USA 2012, 109:2607-2612; Neuron 2013, 77:115-128), temperature circuits (Nature Communications 2015, 6:7775) and established a comprehensive map of the central complex indicating the direction of information flow at single cell resolution (Cell Reports 3:1739-1753, 2013). For his contribution in connectomics, Chiang was invited as one of the Presidential Special Lecturers at the Neuroscience 2016 Annual Meeting where he demonstrated the first effort toward whole body connectomics (reviewed in J Neurosci 2016, 36:11375–11383).
一種提高三維影像資訊密度之方法-Image processing method for feature retention and the system of the same (江安世、張修明、陳永昌、陳冠宇), 中華民國專利證書:I435112, 專利期間:2014/04/21-2031/06/02；US patent No: 8665276, Effective period: 2014/03/04.
Sample carrying apparatus capable of revolving sample (江安世、傅建中、張修明), US patent No: 8659825 B2, Effective period: 2014/02/25.
一種建構和搜尋三維影像資料庫之方法- A Method for Searching and Constructing 3D Image Database(王聖川、江安世、李秉璋、林敬堯、荊宇泰、莊朝鈞、張修明、陳永昌、蔡坤龍、謝昌煥),中華民國專利證書: I406181, 專利權期間:2013/8/21-2029/05/10;US patent No:8520933, Effective period: 2013/08/27.